“After two human clinical studies, ProHydrolase has been proven to maximize the performance benefits of protein supplements”~ John Deaton, Ph.D

Understanding Protein Digestion

Whey protein is one of the richest sources of all nine essential amino acids that the body is unable to produce on its own1. However, whey protein must first be processed into a usable form, which includes essential amino acids and beneficial peptides (i.e., Di and Tri-peptides)2.

To be effective, protein must be broken down into a smaller particle size within approximately 90 minutes of consumption3. This transit time represents the time from which the whey powder passes from the stomach through the small intestine where digestion/absorption primarily occurs; from there it will be assembled into muscle protein (a bio-usable form)3. To visualize this process, think of a large table being moved into a room through a door that is too small. The table must first be broken down to pass through the doorway before it can be reassembled and used. Undigested whey protein will simply be excreted from the body, resulting in wasted amino acids.

Furthermore, when whey protein is not broken down into the smallest composition, it creates large peptides that can cause discomfort such as bloating, nausea and cramping. These symptoms should not be confused with lactose intolerance. Lactose is not present in whey isolates; therefore the underlining cause of the discomfort is peptide sensitivity.


1. Borsheim, E., K. D. Tipton, S. E. Wolf, and R. R. Wolfe. Essential amino acids and muscle protein recovery from resistance exercise. Am.J.Physiol Endocrinol.Metab 283: E648-E657, 2002.

2. Tarnopolsky MA, Atkinson SA, Mac-Dougall JD, Chesley A, Phillips S, Schwarcz HP. Evaluation of protein requirements for trained strength athletes. J Appl Physiol. 1992 Nov;73(5):1986-95.

3. Kim SK: Small intestine transit time in the normal small bowel study. Am J Roentgenol 1968, 104: 522-4.

About ProHydrolase

ProHydrolase is a bio-sufficient supplement designed to build muscle mass and accelerate muscle recovery when used as an ingredient in protein supplements or meal replacements.
Takes full advantage of the availability of essential amino acids for building muscle and improving muscle recovery
Greatly reduces digestive discomfort caused when protein is not broken down into the smallest composition.
Maximizes the performance benefit of protein supplements, assisting the body in absorbing more protein rather than excreting it.
Effective at digesting various forms of protein including whey, soy, egg, casein, hemp and pea.


These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease. The information contained in this website is intended for educational purposes only. If you are currently taking medications, or have other health-related conditions, consult a physician before adding supplements to your diet.

The Science

Numerous in vitro studies plus two separate human clinical studies have shown the superior ability of ProHydrolase to quickly break down protein, increase available amino acids in the blood, decrease C-reactive protein levels and decrease the large peptides associated with stomach discomfort that results from consuming protein.

In vitro Testing: Gel Electrophoresis

Discomfort PeptidesProHydrolase breaks down large peptides, reducing the potential for discomfort that often is a result of consuming protein.
Amino Acids Lost
Undigested whey protein will simply be excreted from the body, resulting in wasted amino acids.


Whey breakdown pancreatin

Plant Proteins



Clinical Results

A full write-up of the clinical study on ProHydrolase is available by request. Contact Deerland Enzymes for information.

Total Amino Acids
Total Amino Acids

C-Reactive Protein


Total amino acid concentrations increased 55mg in the blood over the 270 minutes after ingestion of whey protein isolate with ProHydrolase when compared to taking whey protein isolate alone. This correlates with published results showing the increase of amino acids when taking whey hydrolysate vs. whey protein unprocessed.


CRP levels start at 750 micrograms/liter, and increase to approximately 900 micrograms/liter after consuming whey. With the addition of ProHydrolase, levels decrease to 500 micrograms/liter, and then gradually increase over the 4 hours to 700 micrograms/liter.

Frequently Asked Questions

What is ProHydrolase?
ProHydrolase is a proprietary blend of proteolytic enzymes, designed to build muscle mass and accelerate muscle recovery when used as an ingredient in protein supplements or meal replacements. Benefits include: 1) digests protein into bio-usable form to increase protein absorption, 2) maximizes availability of amino acids, 3) greatly reduces stomach discomfort (nausea, bloating, cramping) associated with consuming high amounts of protein, 4) effective at digesting whey, casein, egg, soy, hemp and pea proteins.

How does it work?
ProHydrolase helps break down protein efficiently and completely so that the body can maximize the absorption of amino acids into the blood stream, and reduce the exposure time to whey peptides that can cause discomfort. Consumption of whey protein with ProHydrolase significantly raises the level of amino acids in the blood, compared to whey isolate alone. Among those amino acids are the branch chain amino acids, which have been shown to play a vital role in muscle synthesis and recovery.

Is it Kosher?
The formula is Kosher, and a copy of the certification is available by request.

Is it Vegetarian or Vegan?
The formula is vegetarian and vegan in accordance with the guidelines of the American Vegetarian Association.

Is it non-GMO?
Yes, ProHydrolase is Non-GMO Project Verified.

What is the recommended dosage?
Dosage: 10mg of ProHydrolase per gram of protein. It is important to note that most containers of protein supplements are not 100% pure protein, so the calculation must be adjusted to account for the amount of pure protein in the container.

Are there clinical studies that prove it works or reference studies we can review?
Two separate human clinical studies have been performed, and numerous reference studies are available. Contact us for more details on these studies.

Is it GRAS?
Yes, the enzymes that make up ProHydrolase are Generally Recognized as Safe (GRAS) by the FDA.

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