"DE111 is clinically proven to support a healthy gut flora by controlling microbial populations and promoting proper digestion of dietary fats and complex carbohydrates."~ John Deaton, Ph.D.

Understanding Probiotics for Digestive and Immune Health

The human body carries nearly 100 trillion bacteria in the gut…that’s more than 10 times the total number of human cells in the entire body. Probiotics are those “good” bacteria that help keep the intestines healthy and assist in digestion and nutrient absorption. Researchers are also finding evidence that certain bacteria in the gut influence the development of aspects of the immune system1,2. In fact, the gut accounts for 25% of the immune cells in the body which provides 50% of the body’s immune response.

Probiotics work to help maintain balance in the intestinal microbiota. By enhancing the intestinal flora, these microorganisms may have a larger effect in terms of keeping people in good health. Understanding the type and quantity of microorganisms in the gut has become a critical goal in the pursuit of overall wellness. Recent research on the microbiome has shown that its influence extends far beyond the gut, playing a crucial role in both our digestive and immune systems. In fact, studies are showing the microbiome’s role in such areas as brain health, memory and even mental health. Consumers today have the ability to influence their gut microbiota like never before– from supplements to food, people are seeking sources of good bacteria.

Probiotic Strains: Spore and Non-Spore Formers
The majority of probiotics currently available are bacteria which are non-spore formers, such as Lactobacilli and Bifidobacteria. These probiotic strains have been widely studied for their health benefits and are a popular choice for use in dietary supplements or cold-processed foods, such as yogurt.

Spore forming bacteria are a diverse group of very hardy bacteria, characterized by their ability to form endospores to protect themselves in varying conditions such as high temperatures and the acidic environment of the gut. The Bacillus subtilis species of microorganism has been known for almost 100 years, having first been isolated and described in 1915. It is considered to be a normal inhabitant of the gut in animals and humans3.

Bacillus subtilis has the ability to form spores that protect the microbes from harsh conditions until they enter an environment ripe for germination, such as the GI tract. Because of this spore-forming ability, Bacillus subtilis offers additional benefits as a probiotic:

  • Remains viable under a wide temperature range, doesn’t require refrigeration4
  • Survives passage through the acidic environment of the GI tract5
  • Can persist in the GI tract increase its numbers and then re-sporulate6
  • Supports the normal immune reaction of intestinal cells7,8
  • Crowds out bacterial pathogens and maintains healthy gut flora9,10
  • Communicates with intestinal cells to maintain gut barrier function11

Each type and strain of probiotic, spore and non-spore forming, performs a different role with particular benefits in terms of digestion and immunity, as well as where in the GI tract they act. Multi-strain probiotic supplements provide a broad spectrum of benefits.

1. Fiorini G, Cimminiello C, Chianese R. II B. subtilis come stimolatore selettivo delle IgA linfocitarie di membrana. Farmaci. 1985;9:331–334.

2. Meroni P L, Palmieri R, Barcellini W, De Bartolo G, Zanussi C. Effect of long-term treatment with B. subtilis on the frequency of urinary tract infections in older patients. Chemioterapia. 1983;2:142–144.
3. Novelli A, Ulivelli A, Reali E F, Mannelli F, Trombi-Belcari L, Spezia R, Periti P. Bacillus subtilis spores as a natural pro-host oral agent. Preliminary data in children. Chemioterapia. 1984;3:152–155.
4. Holtmann, G. & Bremer, E. (2004). Thermoprotection of Bacillus subtilis by exogenously provided glycine betaine and structurally related compatible solutes: involvement of the Opu transporters. J Bacteriol 186, 1683–1693.
5. Leser, T.D., Knarreborg, A. and Worm, J. (2008), Germination and outgrowth of Bacillus subtilis andBacillus licheniformis spores in the gastrointestinal tract of pigs. Journal of Applied Microbiology, 104: 1025–1033. doi: 10.1111/j.1365-2672.2007.03633.x (ACIDIC)
6. Tam, Nguyen K. M. et al. “The Intestinal Life Cycle of Bacillus Subtilis and Close Relatives.” Journal of Bacteriology 188.7 (2006): 2692–2700. PMC. Web. 24 Feb. 2015.
7. Bonomo R, Luzi G, Frielingsdorf A, Aiuti F. Ruolo delle IgA secretorie nelle funzioni dell’immunita locale dell’apparato digerente. Impiego di spore di B. subtilis in alcune forme morbose con deficit di IgA e ipogammaglobulinemia. Chemioter Antimicrob. 1980;3:237–240.
8. Ciprandi G, Scordamaglia A, Venuti D, Caria M, Canonica G W. In vitro effects of Bacillus subtilis on the immune response. Chemioterapia. 1986;5:404–407.
9. Vacca A, Pantaleo G, Ronco M, Dammacco F. Chemoimmunotherapy for multiple myeloma using an intermittent combination drug schedule (melphalan + prednisone) and alternating course of B. subtilis spores. Chemioterapia. 1983;2:300–305.
10. Mazza P. The use of Bacillus subtilis as an antidiarrhoeal microorganism. Boll Chim Farm. 1994;133:3–18.
11. Thomas, Carissa M, and James Versalovic. “Probiotics-Host Communication: Modulation of Signaling Pathways in the Intestine.” Gut Microbes 1.3 (2010): 148–163. PMC. Web. 24 Feb. 2015.

About DE111

Deerland Enzymes has genome sequenced and tested a highly effective strain of Bacillus subtilis, a probiotic spore that works as a complement to many of the non-spore strains on the market today. Numerous in vitro tests along with a human clinical study have show the strain’s ability to:

in the gut, promoting growth of good bacteria and crowding out bad bacteria such as E. coli.
of dietary fats and complex carbohydrates into beneficial small chain fatty acids such as omega-3 and omega-6.
of cholesterol, glucose and triglycerides.


Because DE111 remains viable under a wide temperature range, the probiotic is ideal for use in supplements as well as foods and beverages.

DE111 bottle



Foods and Beverages: Processing Stability

Food Product

Processing Simulation Method

Average % CFU Survival

Green Tea (Hot) A green tea bag containing DE111 was placed in boiling water and steeped for 5 minutes. A cell count was performed on the hot tea. 94%
Chocolate Bar
(70% cacao)
A bar of chocolate was melted to 48°C, DE111 was added. The mixture was cooled to 27°C and heated back to 32°C before the chocolate was poured into molds to set. 98%
Instant Drink Powder,
Chocolate Flavor
DE111 was added to the drink powder and combined with cold milk. A cell count was performed on the prepared drink. 89%
Dairy Processing DE111 was added to 100 mL of skim milk. The milk was heated to 80.6°C for 22 seconds, then immediately chilled and a cell count was performed. 100%


These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease. The information contained in this website is intended for educational purposes only. If you are currently taking medications, or have other health-related conditions, consult a physician before adding supplements to your diet.

The Science


Through Deerland Enzymes’ partnership with Cornell University, DE111 was fully sequenced for safety and has been uploaded to GenBank, the National Institutes of Health genetic sequence database.


A randomized, double-blind, placebo controlled human clinical study has shown the ability of DE111 to support a healthy gut microflora by controlling microbial populations as well as support digestion and maintain general health.

Controls Microbial Populations
DE111 is a classic Bacillus subtilis strain that has the ability to crowd out E. coli and other bacterial pathogens, allowing for a proliferation of beneficial bacteria.


e coli

Supports Digestion and Maintains General Health
DE111 supports the normal breakdown of complex carbohydrates and fats, promoting proper digestion and nutrient absorption.




A complete write-up of the clinical study is available upon request. Contact Deerland Enzymes for more information.

Frequently Asked Questions

What is DE111?
DE111 is Deerland Enzymes’ strain of the probiotic spore, Bacillus subtilis. Probiotics are those “good” bacteria that help keep the intestines healthy and assist in digestion and nutrient absorption. Probiotics are live microorganisms, which, when administered in adequate amounts, confer a health benefit on the host. (Guidelines for the Evaluation of Probiotics in Food, FAO/WHO)

How does it work?
DE111 controls microbial populations in the gut by crowding out bad bacteria such as E. coli, making room for the growth of good bacteria. DE111 can bind to the epithelial cells of the intestinal lining and increase in number; this promotes a stronger intestinal lining to protect against immune responses to food molecules that may be interpreted by the body as foreign objects. DE111 supports digestive and general health by producing enzymes that aid in the digestion of dietary fats and complex carbohydrates into beneficial small chain fatty acids such as omega-3 and omega-6.

What are the base requirements for good bacteria as functional probiotics?
– Identification at strain level of the microorganism by the most current methodology including a combination of phenotypic and genetic tests.
– Strains must be deposited in an internationally recognized culture collection.
– In vitro tests to detect physiological and functional health potentials of the strain.
– In vivo trials to confirm efficacy in humans and animals.

Does DE111 satisfy the base requirements?
Yes, DE111 meets base requirements of probiotics for foods and supplements.
– The DE111 strain of Bacillus subtilis has been characterized and study by multiple universities and external research laboratories: genetically and phenotypically.
Bacillus subtilis was one of the first bacteria to be studied and has been consumed for centuries in Japan (fermented soybeans).
In vitro studies revealed probiotic properties of Bacillus subtilis DE111, such as adhesion and colonizing of the epithelial cells, acting as a competitive exclusion agent against harmful bacteria, surviving passage through acidic environment of the GI tract, and remaining viable under wide temperature range.
– Oral administration of Bacillus subtilis DE111 helps digest and convert sugar and fats, and helps maintain glucose, cholesterol, and triglyceride levels in the blood.

Does the vegetative form inhabit in the intestine?
No, the vegetative form of Bacillus subtilis is transient; this means that the bacterium does not inhabit the intestine permanently.

Is Bacillus subtilis endogenous to the human gut?
Yes; in fact, in the clinical study, many of the participants were found to have Bacillus subtilis in their stool samples prior to beginning the trial.

Is it vegetarian or vegan?
The formula is vegetarian and vegan in accordance with the guidelines of the American Vegetarian Association.

Is it non-GMO?
Yes, it is Non-GMO Project Verified.

What is the recommended dosage?
The recommended dosage is 5 billion CFU/dose.

How many CFUs per gram are in Deerland Enzymes’ DE111?
Deerland Enzymes offers DE111 in 100 billion CFUs per gram.

What is the stability or shelf life?
The DE111 probiotic doesn’t require special storage conditions like many other probiotics. The expected shelf life is 24 months when stored in air tight containers away from light and moisture, at room temperator (25°C). (Optimum stability is obtained when stored at conditions of ≤25°C; ≤40% RH)

What are the suggested delivery forms and applications?
DE111 can be prepared in forms of tablets, capsules, chewable tablets, yogurts and functional foods.

Does it contain allergens, or is it processed with allergens?
No allergens are used in the fermentation or production.

Is it GRAS?
Yes, Bacillus subtilis is Generally Recognized as Safe (GRAS).

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