"By keeping free radicals in check, ThioZymeAO promotes a steady antioxidant/pro-oxidant balance."~ Theodore Hersh, M.D.

Understanding Antioxidants and Free Radicals

Environmental factors, the aging process and even the body’s normal metabolic functions all generate pro-oxidants, which can act as toxins in the body.

Pro-oxidants are free radicals that are byproducts from the body’s use of oxygen in the energy cycle. These pro-oxidants cause the same type of oxidative damage in the body that we observe watching a peeled apple decay and turn brown. Our organs experience the same type of decay and erosion over time from unchecked free radicals1.

Antioxidants are well known for the protection they provide our bodies from damaging free radicals. Free radicals are unstable atoms or molecules that oxidize other molecules, and are produced by our bodies as part of normal metabolism. These oxidized products are even more unstable and react with other molecules in domino-like chain reactions, leading to oxidative stress. Oxidative stress is the total burden placed on organisms by the constant production of free radicals. This stress may result from environmental factors, lifestyle choices, and even the aging process. By the time a free radical chain dies out, it may negatively impact vital components of our cells.

Free radicals are also generated in the cells of the body by the metabolic process of converting food to energy. As a result, the cells have a built-in antioxidant “defense system” that guards against reactive oxygen species (ROS) and toxic free radicals.2 This antioxidant defense system is comprised of many interrelated substances that maintain a proper balance of reduced/ oxidized antioxidants. Evidence implies that maintenance of the body’s natural defense system could reduce development of disease.1

The Balance of Power
Well known components of the antioxidant system include vitamins C and E, alpha-lipoic acid, as well as enzymes such as catalase and superoxide dismutase (SOD). However, the key components to the body’s defense system are glutathione, along with ample supplies of selenium-amino acids and the amino acid cysteine, because of their ability to recycle each other and the aforementioned antioxidants. Glutathione is the most studied and important protective molecule in the human body, while cysteine imparts critical functionality to glutathione.

For example, the conjugate base of alpha-lipoic acid (lipoate) is reduced to dihydrolipoate (DHLA), which is able to attack reactive oxygen species such as superoxide and peroxyl radicals, among others. It also protects membranes by interacting with vitamin C and glutathione, which may in turn recycle vitamin E.3 Alpha-lipoic acid is able to cross the blood-brain barrier and functions in fat or water. In fact, it both interacts with its antioxidant partners — vitamins E and C — and also helps to conserve them. When the body is deficient of glutathione and lipoic acid, the other antioxidants do not properly network together.4

Several types of antioxidants play various roles in the defense network in vivo3. A balance of pro-oxidant/antioxidant levels within this network is critical to proper function of the body’s defense system. Mammalian cells have developed numerous mechanisms to prevent or treat injurious events that can result from normal oxidative by-products of cellular metabolism. The “Glutathione (GSH) antioxidant system” – a critical component of ThioZyme AO – is foremost among these natural protective systems because GSH contributes directly to the destruction of reactive oxygen compounds and maintains, in reduced active form, vitamins C and E, which also exert an antioxidant effect4.

1. Hersh, T. and Barkin, W. The Balance of Power.

2. Hersh, T., To “C” or not to “C.” The Dual Role of Vitamin C as an Antioxidant and Pro-oxidant.

3. Niki, E. Assessment of Antioxidant Capacity in vitro and in vivo. Free Radical Biology & Medicine. 2010. Vol 49, 503–515. Elsevier, Inc.

4. Bounous, G, Molson, J. Competition for Glutathione Precursors Between the Immune System and the Skeletal Muscle: Pathogenesis of Chronic Fatigue Syndrome. Med Hypothesis 53; (4): 347-349.

5. Poveromo, J. Giving Selenium Careful Consideration. Nutrition Industry Executive, June 2010, pp. 44-45.

About ThioZyme AO

Combats free radicals that cause cell damage and accelerate aging.
Unlike most antioxidant products on the market today, the ThioZymeAO formula combines pivotal cellular antioxidants with their vital partners, antioxidant enzymes, to combat toxic free radicals and help alleviate damaging oxidative stress.

Replicates the body’s natural defense system.
The patented Thione Complex™, in combination with other components of ThioZymeAO, work synergistically to keep the body’s defensive network functioning properly, both by direct antioxidant action and by regeneration of antioxidants, including glutathione itself.

AO bottle


These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease. The information contained in this website is intended for educational purposes only. If you are currently taking medications, or have other health-related conditions, consult a physician before adding supplements to your diet.

The Science


anti becomes pro


regen vit c
cell defense regen C
The cooperative effect of antioxidants is both intra- and extra-cellular.

Gluta destruction h2o
In performing its antioxidant function, two GSH are oxidized to GSSG (pro-oxidant). In order for GSH regeneration via glutathione reductase to occur, Selenium (i.e. Selenomethionine) is necessary.

Frequently Asked Questions

What is ThioZymeAO?
ThioZymeAO is an antioxidant formula designed to replicate the body’s own defense network, in order to combat free radicals that cause cell damage and accelerate aging.

How does it work?
ThioZymeAO is a formula of single antioxidants, such as Vitamin C and E, along with antioxidant enzymes and the patented Thione Complex, which contains glutathione and selenomethionine, the prime antioxidants that help maintain cellular integrity and function.

Is it Kosher?
The formula is not Kosher.

Is it Vegetarian or Vegan?
The formula is vegetarian and vegan in accordance with the guidelines of the American Vegetarian Association.

Is it non-GMO?
The microorganisms used to produce this enzyme product are not genetically engineered organisms as defined by the National Organic Standards Board. Genetically engineered is defined as follows:
Made with techniques that alter the molecular or cellular biology of an organism by means that are not possible under natural conditions or processes. Genetic engineering includes recombinant DNA, cell fusion, micro-and macro-encapsulation, gene deletion and doubling, introducing a foreign gene, and changing the position of genes. It shall not include breeding, conjugation, fermentation, hybridization, in-vitro fertilization, and tissue culture.

What is the recommended dosage?
Dosage: 1 capsule, two times a day with meals.

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